Hog cholera virus: molecular composition of virions from a pestivirus

A second envelope glycoprotein mediates neutralization of a pestivirus, hog cholera virus.

Several monoclonal antibodies (MAbs) raised against hog cholera virus (HCV) reacted with the HCV structural glycoprotein gp44/48 and neutralized the virus. The presence of HCV gp44/48 on the viral surface was directly demonstrated by immunogold electron microscopy. Eight anti-HCV gp44/48 MAbs were tested by immunoperoxidase assay against a panel of pestivirus strains. Each MAb showed a distinct...

The structural components of hog cholera virus.

Hog cholera virus grown in PK-15 cells and SK-cells was labeled with [35S]methionine and [3H]uridine. At least 3 polypeptides were resolved by polyacrylamide gel electrophoresis after disruption of the virus with sodium dodecyl-sulfate. The molecular weights of the structural proteins were determined to be 55000 (p55), 46000 (gp46), and 36000 (p36). The molecular weight of the viral RNA was det...

Cultivation of the Hog Cholera Virus

The work of Hecke on the cultivation, of hog cholera virus was confirmed with ease. Virus was grown in the presence of fresh minced swine testicle in flasks containing Tyrode solution, on the chorioallantoic membrane of embryonated eggs, and on the surface of swine serum agar. In flasks it was grown for 14 transfers; while on eggs it was grown for 13 transfers, followed by an equal number of tr...

Modified Live Virus Hog Cholera Vaccine--M.L.V

Hog cholera virus: art and facts.

The aim of this review is to summarize the informations accumulated during the two last decades on hog cholera virus, a member of the pestivirus genus. Different aspects concerning the virus-host cell interactions, and the structural and antigenic properties of the virion itself are successively analyzed. Despite numerous works, many basic informations are still lacking, which can be explained ...